polyacrylamide gel electrophoresis


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Related to polyacrylamide gel electrophoresis: Agarose gel electrophoresis

pol·y·a·cryl·a·mide gel e·lec·tro·pho·re·sis (PAGE),

a gel formed by cross-linking of acrylamide that is used for the separation of proteins or nucleic acids. These substances are separated on the basis of both size and charge.

polyacrylamide gel electrophoresis

A type of high-resolution zone electrophoresis of nucleic acids and proteins, which is performed on a cross-linked polyacrylamide gel.

pol·y·a·cryl·a·mide gel e·lec·tro·pho·re·sis

(PAGE) (pol'ē-ă-kril'ă-mīd jel ĕ-lek'trō-fŏr-ē'sis)
Separation of proteins or nucleic acids on the basis of both size and charge in a gel formed by cross-linking of acrylamide.

polyacrylamide gel electrophoresis

see GEL ELECTROPHORESIS.
References in periodicals archive ?
Since DNA fragments generate a unique migration pattern for each PCR fragment, it is easier to recognize possible contamination, which may not be suspected by polyacrylamide gel electrophoresis or agarose gel analysis, in which the ranges of migration for bands are narrower compared to the TGGE method.
Basic principles of post translational modification of proteins and their analysis using high resolutions two dimensional Polyacrylamide Gel Electrophoresis, In two Dimensional Electrophoresis and Immunological Techniques, Plenum, New York, London.
Accurate results - In mass spectrometry, 1D and 2D polyacrylamide gel electrophoresis and other proteomics applications
After dialysis with distilled water to remove excess salts, the purity of the preparations was monitored by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) (28) and electron microscopy (26).
Haptoglobin phenotype was determined from 10 [micro]L of hemoglobin-enriched plasma by polyacrylamide gel electrophoresis and peroxidase staining (8).
Whole-cell protein samples were prepared, separated by polyacrylamide gel electrophoresis (running length 9 cm), digitized, and subjected to comparative numerical analysis (13).
The LabChip GXII is Caliper's high throughput system for analyzing protein size, concentration, and purity and serves as a replacement for traditional polyacrylamide gel electrophoresis (PAGE).
The 11 dsRNA segments were separated by polyacrylamide gel electrophoresis and stained with silver to identify the two main RNA profiles, or electropherotypes (long and short).
The new variant's monomeric structure has been confirmed by several independent methods, including gel filtration and native polyacrylamide gel electrophoresis.
The PCR products were then separated by 5% polyacrylamide gel electrophoresis for 2 h (17).
The design of this study utilizing ProteEx's very sophisticated two-dimensional polyacrylamide gel electrophoresis with protein patterning has the potential to make a large contribution not only to the general field of proteomics but also specifically for women with breast cancer.
PCR products were purified by polyacrylamide gel electrophoresis and used for sequence analyses.