Yang and colleagues designed two guide RNAs that target the pol gene of the retroviruses for cutting.
That pattern indicates that as Cas9 sliced genes, the cells often repaired the damage by copying a previously edited pol gene.
Genomic sequence comparison and phylogenetic analysis based on amino acids of the pol gene
(2,562 bp) were constructed with ClustalW version 2.
Moreover, the molecular cloning of POL gene
would provide valuable information for further understanding of the structure-function relationship through its expression and mutagenesis.
Ghent, Belgium) has patented a method for detection and/or genetic analysis of HBV in a biological sample, comprising hybridizing the polynucleic acids of the sample with a combination of at least two nucleotide probes, with said combination hybridizing specifically to a mutant target sequence chosen from the HBV RT pol gene
region and/or to a mutant target sequence chosen from the HBV preCore region and/or to a mutant target sequence chosen from the HBsAg region of HBV and/or to a HBV genotype-specific target sequence, with said target sequences being chosen from FIG.
The TRUGENE HBV Genotyping Test sequences the pol gene
region where most mutations occur.
Previous subtyping, based on phylogenetic analyses of pol gene
sequences, showed that sequences from patients TV721 and TV749 grouped together (100% bootstrap) but only loosely with clade J references (61% bootstrap); sequences from patients TV725 and TV919 grouped outside all major clades (6).
Novartis AG (Basel, Switzerland) has patented BIV constructs encompassing BIV combination vectors, BIV vectors and BIV packaging vectors and particularly the invention pertains to a three vector system comprising: a) a BIV vector construct including a DNA segment from a BIV genome, a packaging sequence to package RNA into virions; a promoter operably linked to the DNA segment; and a transgene operably linked to a second promoter; b) a BIV packaging vector construct comprising a BIV DNA sequence fragment comprising at least a gag gene or pol gene
of BIV; a promoter operably linked to the BIV DNA fragment; and a polyadenylation sequence located downstream of the BIV DNA fragment; and c) an expression vector construct comprising a gene encoding a viral surface protein.
HIV-Integrase is a 31 kd protein produced from the the C-terminal portion of the Pol gene
product and is responsible for the integration of proviral DNA into the host genome, which is essential for HIV replication.
In cases in which viral load was >1,000 RNA copies/mL plasma, HIV-1 genotyping was performed by using a protocol (Virco BVBA, Mechelen, Belgium) based on sequencing of a 1,497-bp fragment of the HIV-1 pol gene
The gag and pol genes
are considered to be potentially effective components of a vaccine against multiple HIV-1 mutants.
The major retroviral pathogen of meat-strain chickens is an infectious recombinant between ALV gag and pol genes
and an env gene related to EAV (12).