Just a little scientific fact, eggs contain phosvitin
, which inhibits the absorption of iron; but because I use nuts and seeds, my desserts are very high in iron," Nazmy explained.
Hen egg yolk phosvitin is a phosphoglycoprotein that forms the structure of yolk granules by making phosphocalcic bridges with high-density lipoprotein (HDL) (Taborsky, 1983).
The metal chelating ability of phosvitin enables it to be used as a functional material.
Phosvitin extraction and purification methods reported in previous studies used organic solvents, such as ether, a combination of chloroform and methanol, a combination of hexane and ethanol, or ethanol to remove lipids and phospholipids from yolk granules (Heald and McLachlan, 1963; Wallace and Morgan, 1986; Losso and Nakai, 1994).
Phosvitin and [beta]-HDL are not affected by thermal treatments up to 82 [degrees]C, whereas LDL and a-HDL are denatured at 72 and 76[degrees]C, respectively.
Therefore, we hypothesized that phosvitin could be extracted with salt and purified by thermal treatment without using organic solvents.
Phosvitin standard, acrylamide, N,N'-methylene-bis-acrylamide, tris (hydroxymethyl) aminomethane (Tris), sodium dodecyl sulfate (SDS), ammonium persulfate, N,N,N',N'-tetramethyleethylenediamine (TEMED), glycine, glycerin, Coomassie Brilliant Blue G-250, aluminum nitrate, and Tween 20 were obtained from Sigma-Aldrich (St.
The relative phosvitin composition in the protein solution was estimated from band intensity (pixel intensity x band area).
The iron-binding ability of the isolated phosvitin was determined using a modified ferrozine method (Riemer and Hoepken, 2004).