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phase-contrast microscopy

   Also found in: Encyclopedia 0.01 sec.
phase-contrast microscopy,
a type of light microscopy in which a special condenser and objective with a phase-shifting ring are used to visualize small differences in refractive index as differences in intensity or contrast. It is useful in viewing unstained specimens that appear transparent.


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This reprint of the original 2003 edition covers: elementary principles of phase-contrast microscopy, electron optics, wave optics, coherence and Fourier optics, STEM and Z-contrast, electron sources and detectors, measurement of electron-optical parameters, instabilities and the microscope environment, experimental methods, and associated techniques.
 
 
 
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