2] formed against the organism within the phagolysosome
But the assay is semi--predictive, it neither tests for penetration of the compound into the host cell nor for activity in the peculiar environment of the macrophage phagolysosome
Lysosomal granules also fuse with cytoplasmic phagosomes to discharge lysozyme into phagolysosomes
for killing or digesting trapped microorganisms.
As a next step, we are currently using Caprion's CellCarta platform to identify all the proteins involved in the pathway, from phagosome formation to their transformation into phagolysosomes
, the process required to kill pathogens and initiate an efficient immune response.
In all fixed samples, the following details were evaluated separately: nuclear and nucleolar preservation; chromatin pattern and details; mitosis and chromosomes, if present in the metaphase; cytoplasmic details, such as phagolysosomes
in the alveolar macrophages; and mucin in mucinous adenocarcinomas.
Despite this battery of defense mechanisms, several bacteria and parasitic species survive and even multiply within macrophage phagolysosomes
After internalization of bacteria, the endosome ceases to mature and does not accumulate markers of late endosomes or phagolysosomes
that are unable to digest their contents form the pathognomonic MG bodies.
Some lamellated phagolysosomes
or autolysosomes were also seen with variably electron-dense material.
Q fever is a worldwide zoonosis caused by Coxiella burnetii, an obligate intracellular bacteria which lives the phagolysosomes
of the host cell.
Electron microscopy has shown curved membrane-bound phagolysosomes
containing whorled and parallel lamellar phospholipids in the histiocytes.
We can hypothesize that readily water-soluble metals induce acute effects, whereas less soluble metals on PM collected within phagolysosomes
can slowly leach off and produce long-term health effects.