phagemid

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phagemid

An engineered bacteriophage (cloning vector) derived from a phage and a plasmid, into which foreign single- or double-stranded DNA is inserted to facilitate characterisation of cloned inserts of DNA by allowing it to replicate.
 
Applications
High-resolution restriction mapping, rescue of single-stranded DNA, RNA transcription, sequencing of single- and double-stranded nucleic acids, generation of nested deletions, site-directed mutagenesis, prokaryotic expression, eukaryotic expression.
References in periodicals archive ?
The Breitling family of patents covers the use of full-length pIII phage protein as a scaffold in a phagemid vector, and is one of the fundamental groups of patents for the phagemid display of antibody, antibody fragments and proteins.
The reverse transcription-PCR product was cloned as a SfiI-Not1 fragment into the pCANTAB6 phagemid vector, which produced a myc tag fused to the COOH terminus of the scFv gene.
Originating from the DKFZ (German Cancer Research Center) of Heidelberg, Germany, the Breitling patents cover the use of full-length pIII phage protein as a scaffold in a phagemid vector, and are one of the fundamental group of patents for the phagemid display of antibody, antibody fragments and proteins.
These patents cover the use of full-length pIII phage protein as a scaffold in a phagemid vector, and are one of the fundamental patents for the phagemid display of antibody, antibody fragments and proteins.