phage display


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phage display

n.
A method of using genetically modified bacteriophages to make new proteins and replicate selected proteins, enabling the engineering of antibodies and the development of new drugs.

phage display

A technique that fuses peptides to capsid proteins on the surface of a bacteriophage, which allows screening of libraries of phage-displayed peptides for binding to specific ligands. Identification of the DNA sequence of a selected phage identifies its peptide sequence.

phage display

the display of recombinant PEPTIDES/PROTEINS on the surface of a PHAGE, for example M13, so that displayed proteins can be identified by their interaction with other proteins. The technique involves cloning the gene for the protein in a special phage cloning vector, such that the cloned gene is fused with the gene for a phage coat protein. The gene fusion directs synthesis of a hybrid coat protein, when the vector is propagated in an appropriate bacterial host. Upon assembly of the phages, this protein is inserted into the phage coat and hence displayed on the surface of phage particles. The procedure normally involves the use of PHAGE DISPLAY LIBRARY and rapid screening and selection of desired proteins. Uses include the engineering of a wide range of specific ANTIBODIES.
References in periodicals archive ?
XOMA's extensive antibody expertise includes phage display library construction, antibody discovery, optimization, preclinical and clinical development, regulatory affairs, and cGMP manufacturing.
The researchers' success demonstrates that antibody phage display can be used to select antibodies for pathogen detection--even where traditional methods have proved inadequate.
Avidex can routinely produce high affinity mTCRs and is developing a new protein therapeutic platform based on phage display.
Under the terms of the agreement with Cambridge Antibody, Micromet receives a worldwide licence in respect to all Cambridge Antibody's antibody phage display technology patents, both pending and granted, to develop antibody-based products targeting the EpCAM antigen.
Chugai will use CAT's antibody phage display libraries to enhance the efficiency of antibody-isolating processes for functional analysis and target validation of genes in its drug-discovery programs.
The key steps are the use of phage display to identify the unique protein to act as the super-strong glue, and the subsequent cloning to make larger quantities.
For Dyax, it is the first time it has granted a license for the application of phage display affinity technology for separations applications.
Dyax's phage display technology has powered therapeutic and diagnostic candidate discovery across a wide range of therapeutic areas.
com)-- The program achievements were published in the Journal of Nucleic Acids titled “Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems.
today announce an agreement to expand access and freedom to operate under each other's phage display patents.
Cambridge Antibody Technology (Cambridge, England) and Dyax Corporation (Cambridge, MA) announce an agreement to expand access and freedom to operate under each other's phage display patents.
a privately held oncology company, announced today that Dyax has granted Eclipse an exclusive worldwide license for the development and commercialization of preclinical fully-human antibodies discovered using Dyax's gold-standard phage display technology for use in oncology indications.