5, colloidal iron, mucicarmine, periodic acid-Schiff
(PAS), and zirconyl hematoxylin.
There were numerous yeastlike organisms seen with this stain but not with the periodic acid-Schiff
Results of staining with periodic acid-Schiff
, Gram, and Grocott Gomori methenamine silver were negative.
However, to highlight the fungal wall, staining with periodic acid-Schiff
(PAS) (figure 3, B) or Gomori methenamine silver (GMS) will result in a dramatic bright magenta (PAS) or black (GMS).
We agree that Grocott methenamine silver, Congo red, and periodic acid-Schiff
usually stain cellulose; however, modified cellulose, such as cellulose acetate may not stain with periodic acid-Schiff
Duodenal biopsy specimens were negative by periodic acid-Schiff
staining, specific immunohistochemical analysis, and PCR.
The tumor cells contain glycogen, which is highlighted with a periodic acid-Schiff
(PAS) stain (figure 2).
4) The organisms are readily apparent with the use of special histochemical stains, such as Gridley fungus stain, periodic acid-Schiff
(with or without diastase), and Gomori methenamine-silver stain (Figure 3).
The granulation tissue biopsy was positive on periodic acid-Schiff
However, direct microbiologic confirmation of BCG infection was lacking because cultures were negative and Ziehl-Neelsen and periodic acid-Schiff
staining did not show acid-fast bacilli, other bacteria, or fungi in these specimens.
A positive periodic acid-Schiff
with and without diastase and negative iron stain suggest that the pigment was lipofuscin, which has not been previously described, likely secondary to ischemia.
The duct- or gland-like lumens may contain eosinophilic proteinaceous material (which is reactive to periodic acid-Schiff