These include Edrnan degradation, peptide mapping
, and mass spectrometry to compare the amino acid sequences, an in-vitro proliferation bioassay to assess potency, surface plasmon resonance to study target binding, and protein concentration content determination.
Protein surface topology-probing by selective chemical modification and mass spectrometric peptide mapping
UNIFI, a solution initially developed for intact protein mass analysis, peptide mapping
and supporting general bioseparations, has been extended to support a mix of quadrupole time-of-flight (Q-Tof) MS and optical detection instruments within a networked lab workgroup.
We used tryptic peptide mapping
to confirm this supposition and to obtain a more accurate measurement of the decrease in mass.
of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry.
Unfortunately, phosphorylated peptides were not observed; therefore, the peptide mapping
could not confirm the mono-and bisphosphorylation of cTnI that is suspected based on the relative molecular masses of the intact protein.
The epitopes of MAbs 5E4 and H117 have been mapped to the N-terminal region of PSA (amino acid residues 3-11) by synthetic peptide mapping
and cross-inhibition studies (16, 20).
ProFound: an expert system for protein identification using mass spectrometric peptide mapping
The new columns greatly expand our current bio-columns portfolio, which also include reverse phase columns for intact protein separations and peptide mapping
Sequence information is especially important for the identification of proteins and may be obtained by MALDI MS analysis together with the use of enzymatic digestion followed by postsource decay of the resulting peptides or database-based peptide mapping