peptide mapping


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peptide mapping

A means of identifying proteins electrophoretically after partially hydrolyzing them. Each protein leaves a characteristic pattern of light and dark peptide bands on the electrophoretic paper or gel.
See also: mapping
References in periodicals archive ?
These include Edrnan degradation, peptide mapping, and mass spectrometry to compare the amino acid sequences, an in-vitro proliferation bioassay to assess potency, surface plasmon resonance to study target binding, and protein concentration content determination.
Protein surface topology-probing by selective chemical modification and mass spectrometric peptide mapping.
UNIFI, a solution initially developed for intact protein mass analysis, peptide mapping and supporting general bioseparations, has been extended to support a mix of quadrupole time-of-flight (Q-Tof) MS and optical detection instruments within a networked lab workgroup.
We used tryptic peptide mapping to confirm this supposition and to obtain a more accurate measurement of the decrease in mass.
Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry.
Unfortunately, phosphorylated peptides were not observed; therefore, the peptide mapping could not confirm the mono-and bisphosphorylation of cTnI that is suspected based on the relative molecular masses of the intact protein.
The epitopes of MAbs 5E4 and H117 have been mapped to the N-terminal region of PSA (amino acid residues 3-11) by synthetic peptide mapping and cross-inhibition studies (16, 20).
ProFound: an expert system for protein identification using mass spectrometric peptide mapping information.
The new columns greatly expand our current bio-columns portfolio, which also include reverse phase columns for intact protein separations and peptide mapping.
Sequence information is especially important for the identification of proteins and may be obtained by MALDI MS analysis together with the use of enzymatic digestion followed by postsource decay of the resulting peptides or database-based peptide mapping (21-23).