The complete corpus callosum was microdissected from PBS-perfused mice 14 days after progesterone pellet implantation.
In the progesterone pellet implantation group, after two weeks of hormone administration, there were significant increase in MBP (0.
Chronic inflammation was induced by cotton pellet implantation , this protocol was described by Sheth et al.
The first group served as control and the animals received 5% Polysorbate 80 single intragastrically in comparable volumes to drugs used for 7 consecutive days from the day of cotton pellet implantation.
On the other hand, the implantation of foreign body for relatively longer time as in case of cotton pellet implantation model of chronic inflammation promotes the formation of granuloma.
The latter revealed that cotton pellet implantation caused mild degeneration changes in gastric glands.
For the pellet implantation procedure, anesthesia was induced by continuous administration of isoflurane using an open circuit system with a scavenger/recapture system.
Our pellet implantation groups included Ta (negative control), WA (both a low- and high-dose group), and Ni (positive control).
High-dose WA rats euthanized 1 and 3 months after pellet implantation also exhibited significantly elevated spleen:body weight ratios compared with the appropriate Ta-implanted control rats (Tables 2 and 3).
Statistically significant increases in red blood counts, hemoglobin, and hematocrit levels were observed in high-dose WA animals as early as 1 month after pellet implantation and persisted throughout the experimental period (Tables 2 and 3).
and control vehicle were administered orally for 7 consecutive days from the day of cotton pellet implantation