Polygalacturonase (PG) acts on the pectin chain by adding a water molecule to break the linkage between two galacturonan molecules; Pectin lyase (PL) acts on the galacturonan chain by removing a water molecule from the linkage to break it and to release its products; whereas, Pectin esterase esterifies the pectin by removing methoxy esters (3).
Bacillus cereus GS-2 isolated from fruit industrial dump site effectively produces pectin lyase (PL) and Polygalacturonase (PG).
Pectin lyase (PL) activity was assayed by conducting the enzymatic reaction carried out in the following conditions 40[degrees]C; 6pH; length of light is 1cm and optical density at 235nm.
All isolates were submitted for screening for the production of cellulase, pectin lyase and polygalacturonase.
Polygalacturonic acid (for MP5 media) was added as a substrate to determine the polygalacturonase (PG) activity, and mineral medium plus pectin was added as a substrate (for MP7 media) to determine the pectin lyase activity (HANCKIN; LACY, 1984).
CMC- carboxymetilcellulase, PG: polygalacturonase, PL: Pectin Lyase.
Lyases that act on polygalacturonic acid known as pectate lyase, while those act on pectin called pectin lyase, which are widely distributed in various families of microorganisms  and are produced by bacteria especially Bacillus sp.
from soil samples of Damascus, and then focus on optimizing the production and enzymatic characterization of extracellular pectin lyase by Bacillus subtilis strain RSY7.
Pectin lyase production by a Penicillium italicum strain.
Purification and characterization of pectin lyase from Rhizoctonia solani.
Firstly, pectinase is not a single enzyme, but, rather, a set of several pectin-splitting substances: pectinesterases, polygalacturonases, pectin lyases
, and pectate lyases.
This fungus synthesizes polygalacuronases, polymethyl galacturonases, pectin lyases
and pectin esterases.