To the Editor: Nocardia species are Gram-positive partially acid-fast Bacilli that are found in soil and water.
Laboratory examination of the pus showed Gram-positive Bacilli, and a modified Ziehl-Neelsen stain showed partially acid-fast Bacilli without true branching.
belong to a diverse group of bacteria known as aerobic actinomycetes, characterized as being Gram-positive rods with partially acid-fast, non-motile, filamentous branches; other properties include being catalase-positive and methenamine silver-positive.
Smears performed from the colonies revealed Gram-positive microorganisms; partially acid-fast stains (modified Kinyoun acid-fast) were also positive (Figure 2) but Kinyoun stains were negative in all isolates.
It may appear to be Kinyoun's negative, and partially acid-fast
, leading to a potential misidentification as a Nocardia species, as happened in this case.
Cryptosporidium oocysts exhibit acid-fast staining and must be differentiated from partially acid-fast
organisms including Cyclospora cayetanensis .
The organism presents the shape of partially acid-fast bacilli (PAFB).
equi by Prescott, (6) and most isolates proved to be partially acid-fast.
equi, as a first step in the search for partially acid-fast bacilli based on the determination of mycolic-acids by TLC technique, followed by the CAMP test and PCR using the COX primers that amplify the choE gene (Fig.
Species of the genus Rhodococcus (order Actinomycetales, family Nocardiaceae) are aerobic, gram-positive, partially acid-fast
, coccoid to rod-shaped bacteria (9).
Gram stain (Figure, C) and a modified acid-fast stain (Figure, D) revealed gram-positive, partially acid-fast
filamentous organisms branching at right angles within the eosinophilic material consistent with Nocardia organisms.
The organisms are weakly gram-positive and partially acid-fast