FFL(Firefly luciferase) catalyzes the oxidation of luciferin in the presence of ATP, Mg2+ and molecular oxygen to produce light, oxyluciferin
, CO2 and AMP .
ATP also happens to be a key energy source in the enzymatic luciferase reaction, where the substrate luciferin is converted to oxyluciferin
plus emission of a photon.
Through a sequence of another 2 reactions, the released pyrophosphate is converted into adenosine triphosphate (ATP), a cofactor for the enzyme luciferase, oxidizing luciferin to oxyluciferin
2] [light arrow] oxyluciferin
+ PPi + AMP + C02 + light.
2+], catalyzes the oxidation of luciferin by molecular oxygen, yielding the excited state of oxyluciferin