In this study, to improve the efficiency of SCNT in Banna Mini-pig Inbred Line (BMI), we obtained the recombinant nucleoplasmin (Npm) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA).
The ability of nucleoplasmin to decondense sperm is shared by other highly charged polyanionic molecules such as polyglutamic acid, but only nucleoplasmin can cause decondensation at a physiological rate and at a physiological concentration (Philpott et al.
In conclusion, we successfully obtained the recombinant nucleoplasmin by prokaryotic expression system.
Nucleoplasmin facilitates reprogramming and in vivo development of bovine nuclear transfer embryos.
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
Characterization of the ooplasmic factor inducing decondensation of and protamine removal from toad sperm nuclei: involvement of nucleoplasmin.
Nucleoplasmin remodels sperm chromatinin Xenopus egg extracts.
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.