nuclease


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nuclease

 [noo´kle-ās]
any of a group of enzymes that split nucleic acids into nucleotides and other products.

nu·cle·ase

(nū'klē-ās),
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides by cleaving phosphodiester linkages. For nucleases not listed here, see the specific term. Compare: exonuclease, endonuclease.

nuclease

/nu·cle·ase/ (noo´kle-ās) any of a group of enzymes that split nucleic acids into nucleotides and other products.

nuclease

(no͞o′klē-ās′, -āz′, nyo͞o′-)
n.
Any of several enzymes, including the endonucleases and the exonucleases, that hydrolyze bonds between nucleotides in nucleic acids.

nuclease

An enzyme that catalyses the hydrolysis of the phosphodiester bond of polynucleotide chains and phosphate-deoxyribose bonds within (endonuclease) or at the end (exonuclease) of a nucleotide sequence (nucleic acid). Nucleases are produced by most biologic systems and usually recognise a specific substrate, such as single- or double-stranded DNA or RNA.

nu·cle·ase

(nū'klē-ās)
General term for enzymes that catalyze the hydrolysis of nucleic acid into nucleotides or oligonucleotides.
Compare: exonuclease, endonuclease

nuclease

Any one of several enzymes that break down NUCLEIC ACIDS.

nuclease

any enzyme that promotes hydrolysis of NUCLEIC ACIDS. For example, DNase, which catalyses the breakdown of DNA into individual DNA NUCLEOTIDES. The enzyme cleaves PHOSPHODIESTER BONDS of the nucleic acid. See ENDONUCLEASE, EXONUCLEASE.

nuclease

any of a group of enzymes that cleave or digest nucleic acids into fragments or single nucleotides.

S1 nuclease
a single-strand-specific endonuclease that degrades DNA and RNA to nucleoside 5′-monophosphates. The enzyme is a single polypeptide chain of molecular weight 32,000 and is isolated from 'takadiesterase', a digestive enzyme preparation from Aspergillus oryzae. Widely used in molecular biology to map the position of mRNA to its DNA, and also used to remove single-stranded tails from DNA fragments, to produce blunt ends to open up hairpin-loop structures.
References in periodicals archive ?
Tandem overproduction and characterisation of the nuclease domain of colicin E9 and its cognate inhibitor protein Im9.
In conclusion, DNA pooling with the 5' nuclease assay is a highly accurate and potentially inexpensive method for allelotyping.
This newly issued patent provides the company with broad claims to many of the methods used by DNE nucleases to modify eukaryotic cells.
To explore whether the metal inhibition observed was universal to Ape1 nuclease activity, we examined the impact of the environmental metals on Ape1 3'- to 5'-exonuclease function (Wilson 2003).
ZFNs, invented in the mid-1990s, are artificial constructs made of two types of protein: a "zinc-finger" structure that can be designed to bind to a specific short DNA sequence, and a nuclease enzyme that will cut DNA at that binding site in a way that cells can't repair easily.
Since 2009, SAGE Labs has been using targeted nucleases, including CRISPR/Cas9 and zinc finger nucleases, to perform genetic engineering in mice, rats, and rabbits as part of their SAGEspeed custom model generation service.
The team first identified a protein that had both a ubiquitin-binding domain and a known nuclease domain.
These materials and substitutions constitute an important aspect of Precision's genome engineering technologies, known collectively as the Directed Nuclease Editor or DNE.
The rules of virus isolation are the following: 1) the sedimentation rate (based largely on particle size) falls in a specific range; 2) the banding density in a gradient falls in a specific range; 3) the genome is protected from nuclease attack until the protein (+/- lipid) coat is disrupted; and 4) the major proteins present have sequences that agree with at least part of the genome.
Cellectis has already integrated TAL effector nucleases into its DNA Nuclease production platform.
HIV Protected Autologous Zinc Finger Nuclease Driven CCR5 Modified CD4 T-Cells CCR5 (SB-728-T) Reduce HIV Viral Load in CCR5 [eth]o32 Heterozygote Subjects During Treatment Interruption (TI): Correlates of Effect, and Effect of Cytoxan Pre-Conditioning Regimen; [Abstract #283]; Session: "Results of Preclinical and Clinical Development for Hematological and Immunological Diseases,"Thursday, May 22, 2014.