curcas seed was determined using Technicon Amino Acid Analyzer (TSM-1 Technicon Instrument, Basingstoke, Hampshire, UK) using Norleucine
as internal standard.
Amino acid content was determined, following acid hydrolysis (6 N HCl), on a Biochrom 20 amino acid analyzer, using the synthetic amino acid Norleucine
as an internal standard.
7; Scientific Software) using norleucine
(Sigma) as an internal standard.
Biochrom recommends that the extracts are deproteinised with sulphosalicylic acid solution containing Norleucine
as the internal standard.
Oxalic acid, citric acid, tartaric acid, lithium hydroxide, potassium perchlorate, guanidine thiocyanate, D-tert-leucine, norleucine
, [gamma]-aminobutyric acid (GABA), taurine, citrulline, glutamine, asparagine, and amino acid standard mixture (AA-S-18) were from Sigma.
Senetek's pipeline of products for the treatment of sexual dysfunction includes a portfolio of 17 novel VIP analogs, one of which, Stearyl Norleucine
VIP (SNV) is ready to enter Phase I trials.
Samples were hydrolysed for 24 h at 110[degrees]C with 6 M HCl containing 2 g/L reagent grade phenol and 5,000 nmol norleucine
(internal standard) in evacuated and sealed ignition tubes.
INCI: Hexanoyl dipeptide-3 norleucine
acetate (and) lecithin (and) glycerin (and) phenoxyethanol (and) aqua/water