Significance of detecting insulinlike growth factor binding protein-1 in cervicovaginal secretions: comparison with
Nitrazine test and amniotic fluid volume assessment.
The diagnosis of PPROM is made by obtaining a history of leaking amniotic fluid, clinical assessment, including speculum examination, and laboratory tests such as
nitrazine and fern tests.
CHICAGO -- The placental alpha-microglobulin-1 test is superior from a cost-benefit perspective to the combined traditional diagnostic test of pooling,
nitrazine, and ferning for screening in the setting of questionable preterm premature rupture of membranes, Dr.
The physical exam should be delayed until irrigation reaches an endpoint of neutral pH, as measured with
Nitrazine paper.
To verify the premature rupture of membranes in both groups, the speculum, fern, and
nitrazine tests were done.
I prepared the equipment I needed for the three part assessment (pooling,
Nitrazine, ferning).
This is important because a number of POC tests are not always recognized as "tests" by clinicians (eg, hemoccult,
nitrazine pH, provider-performed microscopy).
Use
nitrazine paper to assess the fluid collected from the posterior fornix for alkaline pH; this method has a positive predictive value (PPV) of 99% and negative predictive value (NPV) of 96%.
Fig-leaf ferning and positive
nitrazine testing: semen as a cause of misdiagnosis of premature rupture of membranes.
Premature rupture of membranes was confirmed by vaginal pooling, a positive
Nitrazine test and amniotic fluid arborization.
Nurse O'Connor performed a
nitrazine test and confirmed the presence of amniotic fluid, a sign that the patient could have had premature rupture of membranes.
Cooper's method was slightly more accurate than the 93% rate of
nitrazine strip testing, in which false positives can result from specimen contamination with urine, blood, semen, or vaginal discharges caused by infection.
