nitrate reduction test

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Related to nitrate reduction test: Oxidase test, urease test

nitrate reduction test

A test used in clinical microbiology to detect nitrate reductase, produced by some intestinal bacteria (e.g., Eubacterium lentum, Bacteroides fragilis) and some strains of Veillonella.


any salt of nitric acid. High intakes by animals may occur when they eat large quantities of lush herbage that has been heavily fertilized with a nitrogen fertilizer such as potassium or ammonium nitrate. It can also occur after a long drought during which nitrification bacteria accumulate large amounts of nitrate in the soil and the first growth after the drought breaks may also be toxic.
Pigs fed on food residues may ingest potassium nitrate used in meat curing when their swill contains butcher's residues.

nitrate explosives
nitroglycerin and others used to blast out water holes and left in the hole may cause subsequent nitrate poisoning.
nitrate poisoning
unusual unless animals get access to unguarded fertilizer or butcher shop residue containing potassium nitrate. Causes gastroenteritis with diarrhea and vomiting. Long-term, low level intake thought to cause abortion in cattle. Chief importance is as a constituent of ruminant diet and its conversion in the rumen to nitrite causing nitrite poisoning.
nitrate reduction test
a biochemical reaction used in the identification of bacteria, particularly mycobacteria. It is based on the reduction of nitrate to nitrite and/or the release of nitrogen gas.
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It was found to be positive for glucose, lactose and sucrose fermentation (red colour appeared in test tube) but found to be negative for catalase test (no bubbles were formed), indole test (green layer is formed at the top of test tube), nitrate reduction test, citrate test (green-forest colour in test tube), methyl red Voges-Proskeur test (no red colour appears in test tube), casein hydrolysis test and for starch agar test (no clear zone formation) as shown in Table 1.
0 mm found to be positive for catalase test (bubbles were formed on glass slide), nitrate reduction test and for fermentation tests of glucose, lactose and sucrose (red colour appeared).
6), for nitrate reduction test (no red color formed in test tube), casein hydrolysis test (clear zone was not formed) and for starch agar test (no clear zone).