Ninhydrin reaction | definition of ninhydrin reaction by Medical dictionary
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a test for proteins, peptones, peptides, and amino acids possessing free carboxyl and α-amino groups that is based upon the reaction with triketohydrindene hydrate; a blue color reaction is used to quantitate free amino acids (for example, after hydrolysis and separation of the amino acids of a protein).
nin·hy·drin re·ac·tion (nin-hī'drin rē-ak'shŭn)
A procedure for proteins, peptones, peptides, and amino acids possessing free carboxyl and α-amino groups that is based on the reaction with triketohydrinene hydrate; a blue color reaction is used to quantitate free amino acids.
References in periodicals archive
Quantitative monitoring of solid-phase peptide synthesis by the ninhydrin reaction
Three extracts were hydrolysed in the standard manner (in 6N HCl for 24 hours under vacuum), and the amino acids detected quantitatively using the very sensitive ninhydrin reaction
The Isolab phenylalanine test kit is a modification of a fluorometric procedure involving a ninhydrin reaction
, as published by McCaman and Robins .
Although the analytical approach is very conventional, AAs being separated with a cation-exchange resin and their concentration determined with the ninhydrin reaction
, the instrument provides for extemporaneous automatic mixing of the detection reagent.