To induce myotubule formation prior to performing glucose uptake assays, C2C12 cells were cultured for an additional two days in DMEM containing 2% horse serum (Highveld Biological, Johannesburg, South Africa) at 37[degrees]C in humidified air and 5% [CO sub.
For the glucose uptake assay C2C12 myotubules and Chang cells were starved for 30 min using serum free media containing 0.
Glucose uptake activity in C2C12 myotubules treated with SBI extract at different doses yielded significant (p < 0.
The cell lines selected for in vitro screening of SB1 were C2C12 skeletal myotubules and Chang cells.
The satellite cells readily respond to regenerative cues such as exercise, or injury by proliferating into myoblasts, which will divide a limited number of times before terminal differentiation and fusion to form multinucleated myotubules
(Wager and Conboy, 2005).