To induce myotube
differentiation at 90% confluency, the differentiation medium containing 0.
13] reported that Cr increases the expression of myosin heavy chain and induces muscle-specific protein synthesis in both skeletal and cardiac chicken myotubes
Furthermore, the absence of myogenin reduces MHC expression and myotube
formation, indicating that myogenin has a unique function in the transition from a determined myoblast to a fully differentiated myotube
(Davie et al.
The aim of this study was to determine whether propofol could trigger abnormal calcium fluxes in human myotubes
isolated from malignant hyperthermia susceptible patients harbouring the native ryanodine receptor type 1 mutation.
Confluent (90%) myoblasts were differentiated into myotubes
by culturing the cells in differentiation medium (DM) with Dulbecco's modified Eagle's medium supplemented with 2% horse serum.
Experiments were performed in differentiated myotubes
6-7 days after seeding.
Minet and Gaster  demonstrated that replicative senescence results in decreased mitochondrial mass and ATP content in myotubes
while mitochondrial ATP synthesis rate normalized to mitochondrial protein was unchanged.
formation was achieved after 4 days of incubation, and the cells were used for subsequent experiments.
Myostatin reduces Akt/TORC1/ p70S6K signaling, inhibiting myoblast differentiation and myotube
The Effects of Astragali Radix Extracts on mitochondrial function in C2C12 myotubes
2001) Mediation of IGF-1-induced skeletal myotube
hypertrophy by PI(3)K/Akt/mTOR and PI(3)K/Akt/GSK3 pathways.
Smad signaling is required for myostatin-mediated inhibition of Akt/mTORC1 signaling, myotube
atrophy, and TGF-[beta]--induced fiber atrophy (Goodman and Hornberger 2014; McFarlane et al.