Muscle endurance was measured as fatigue resistance using the same myograph
and set up as for the measurement of strength.
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Methods: Strips of porcine detrusor were prepared in Krebs solution and contractility was measured in a myograph system chamber aired with [O.
Porcine detrusor strips were mounted in a 6 ml tissue bath chamber (610M, Muscle Strip Myograph System, DMT, Denmark, supplied by ADInstruments GmbH, Spechbach, Germany) filled with Krebs solution, serated with 95% [0.
pinnatum leaf press juice (BPJ) and its chemical components in several dilutions (undiluted, 1-10%) on myometrium strips hang up in a myograph chamber.
The contractions of the preserved and clamped strips were taken up by the myograph DMT800MS (Muscle Strip Myograph system.
The contractility of MA was determined by dual wire myograph.
Resistance MA rings (~250 [micro]m internal diameter) were fitted with two stainless steel wires (40 [micro]m internal diameter) and mounted in a dual-channel Mulvany-Halpern myograph (DMT A/S, Model 410A, Aarhus, Denmark) for measurement of isometric tension.
5 mm in length were cut from each artery and then mounted with 20[mirco]m steel wires to separate 5 ml tissue baths of the integrated myograph
system for tension recording.
Intestinal response was recorded isotonically using isotonic myograph
transducer (NARG Bio-systems INC) and physiograph (NK III).
The force of contraction was measured with an isometric force-displacement transducer (F 60 Myograph
, Narco-Biosystems) connected to the upper hook.