conidia were grown in the Eggin's and Pugh medium with 2% xylose and 0.
36) We mutagenized
worms under various conditions, cultured worms on selection media, and isolated many alleles.
cells were collected by centrifugation, washed, and resuspended in one ml freeze-away TSB medium.
Information on the quantum of induced polygenic variability or micromutations and the genetic parameters for different polygenic traits in segregating generations of mutagenized
populations gives an indication about the scope of improvement in these traits through selection (Sheeba et al.
We have also mutagenized
phytoactive compound-producing strains, which greatly improved their production, occasionally by over 100%.
To determine the factors involved in plant growth promotion by this organism, we mutagenized
wild-type strain B16 using OmegaKm elements and isolated one mutant, K818, which is defective in plant growth promotion, in a rockwool culture system.
e cnx and niaD mutants with RS-phenotype Confirmed chlorate sensitive but 18 17 bromate resistant mutants (brn strains with SR phenotype) Confirmed chlorate and bromate 7 12 resistant mutants (cbrn strains with RR phenotype) Mutagenized
strains were inoculated into chlorate master plates (glucose supplemented minimal medium, pH 6.
To make their discovery, the scientists exposed a passel of worms (Caenorhabditis elegans) to a chemical that causes random changes in its DNA, and looked among the mutagenized
worms for those appearing to have a slow rate of metabolism, manifested in their slow development and slow behavioral responses.
The presence of chitin binding domain (ChBD), and absent of cellulose binding domain (CBD) in Chi-a, 131 and in the mutagenized
Seeds homozygous for ser1 were mutagenized
with EMS, germinated and grown to maturity.
The chimeric construct was engineered into a yeast expression vector pKB-KF2 which was subsequently mutagenized
to produce site-directed mutations such as KF3p, KF5p, and KF6p.
The PCR-ELISA used as the comparison method is a competitive PCR based on coamplification of the viral target and of a mutagenized
competitor target that acts as internal control.