cells were collected by centrifugation, washed, and resuspended in one ml freeze-away TSB medium.
2010-Optimization of fermentation conditions of a mutagenized
A large number of wild type Arabidopsis seeds (CS 1092; Lehle Seeds, Round Rock, TX) have been mutagenized
with ethyl methane sulfonate at a range of concentrations.
Since the rare progeny will be heterozygous for the mutagenized
chromosome arm, they are used to isolate a stock of the putative meiotic mutant through a subsequent series of crosses.
Studies analyzing mutagenized
worms have uncovered a role for the calciumactivated large conductance BK potassium channel in ethanol sensitivity (Davies et al.
Diversa Corporation (San Diego, CA) has patented a directed evolution process comprising novel methods for generating improved progeny molecules having desirable properties, including, for example, a method for rapid and facilitated production from a parental polynucleotide template, of a set of mutagenized
progeny polynucleotides wherein at least one codon encoding each of the 20 naturally encoded amino acids is represented at each original codon position.
This line (3-18H) was mutagenized
and genetic screens were conducted on the resulting strain (35S-IRT1) to identify cadmium resistant mutants.
Passing populations of mutagenized
yeast over filters with uniform diameter cylindrical pores that are slightly larger than the average diameter of a yeast cell gives a [greater than]200-fold enrichment of mutants that fail to separate mother and daughter cells through successive generations.
Furthermore, the expression of mutagenized
JAKs can help evaluate physiological function of modified JAKs in signal transduction.
merA sequence, MerApe9, modifying the flanking region and 9% of the coding region, was constructed and placed under the control of plant-regulatory elements.
To address the potential role for the histone H3 core domain in telomeric heterochromatin, the gene encoding H3 (HHT2) was randomly mutagenized
by PCR mutagenesis, and the mutated copies were introduced into yeast lacking the endogenous copies of HHTI and HHT2.
Strain improvement techniques led to mutagenized
microorganisms that produce enzymes in large quantities.