Transposon-mediated reporter gene mutagenesis
has been used extensively in the field of bacterial pathogenesis to identify loci upregulated by mammalian body temperature and other environmental cues such as calcium concentration (4).
played an important role in the solution of the structure in that the cysteine mutants C865, C1 14S, and C173S were used to help locate the positions of heavy atoms and to align the sequence with the model derived from the experimental phases.
Ixsys' Directed Evolution technology is centered around the Kauffman and Codon-Based Mutagenesis
(CBM) patents, which form the foundation of the company's broad discovery and protein engineering capabilities.
The currently prominent approach, generation of novel serotypes from the known cardiotropic ones (AAV6 and 9) by random mutagenesis
, alters favorable serotype attributes and most importantly tropism.
com)-- Creative BioMart, a world leading Biotech Company, has conducted a morning talk to discuss its mutagenesis
Using APOBEC's distinctive DNA mutational signature, the team of researchers examined approximately 1 million mutations in 2,680 cancer samples, and found that, in some tumors, nearly 70 percent of mutations in a given specimen resulted from APOBEC mutagenesis
Transposagen's project titled: Creation of Hyperactive Transposons for Mutagenesis
in Rodents will further develop Transposagen's piggyback (TM) DNA Modification System.
Palmitoylation of DAT by PAT enzymes will be confirmed by site-directed mutagenesis
of DHHC enzymes to determine the specificity, followed by analysis of palmitoylation effects on DAT function.
Among their perspectives are inhibiting HIV entry, helicase-primase inhibitors as a new approach to combating herpes simplex virus and varicella zoster virus, alkoxyalkyl ester pro-drugs of antiviral nucleoside phosphate and phosphonates, maribavir as a novel benzimidazole ribonucleoside for preventing and treating cytomegalovirus diseases, lethal mutagenesis
as an unconventional approach to combating HIV, silencing viruses with antiviral RNAi, and neuraminidase inhibitors as anti-influenza agents.
Smaller projects designed to develop novel techniques, such as strategies for mutagenesis
, conditional gene expression, or for identifying new genes or mutants, as well as new tools to mine and analyze sequence data:
The utility of this vector lies in its ability to isolate, manipulate and maintain large fragments in bacteria and yeast, allowing for mutagenesis
by yeast genetics and simplified preparation of plasmid DNA in bacteria.
and cell transformation in cell culture.