There are significant technical and professional costs associated with the processing and histopathologic analysis of multiple tissue sections with multiple stains
for each lymph node.
This capability allows the clinician to not only do more with a smaller sample, but will also allow the use of multiple stains
on the same sample, therefore increasing the accuracy of the diagnosis.
We have evaluated multiple stains
and we have perfected the Disintegration Detection Stain to be able to isolate brain cell death.
are now applied across a number of slides, and all must be reviewed and compared, and intensities must be quantified with consistency and accuracy.
The double immunohistochemical stain for MUC4/p53 can be a useful diagnostic tool in conjunction with the hematoxylin-eosin-stained section for pancreatic adenocarcinoma, particularly when limited tumor is available for multiple stains.
The rationale for developing the double stain in this study was that there is not always sufficient tissue available for multiple stains when performed on different slides.
This novel method of stain quantification enables several improvements over existing methods including: 1) the detection of tissue on a histological slide by automated robotic microscopes; 2) improvements to auto-focus algorithms ensuring that automated digital imaging of histological slides is confined to tissue areas only; 3) improvements to automated image segmentation and the identification of tissues and their structural features; 4) improvements to the identification of individual stains when multiple stains
are used, and 5) improvements to the quantification of the extent of staining.
Throughput is greatly reduced, however, with non-multiplex-enabled systems since they require the IHC process to be repeated two or more times to achieve multiple stains