multilocus sequence typing


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mul·ti·lo·cus se·quence typ·ing

(MLST) (mŭl'tē-lō'kŭs sē'kwĕns tīp'ing)
A strain typing method that uses polymerase chain reaction to amplify several different genetic loci. The resulting fragments are separated by electrophoresis and analyzed.
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Subsequent whole-genome sequencing (WGS) and core-genome multilocus sequence typing (MLST) (7) resulted in the assignment of 57 human isolates to the unique cluster type 1248 (sequence type 8 according to the Institut Pasteur MLST database; http://bigsdb.
This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using multilocus sequence typing (MLST).
The clinical and flour isolates grouped together, with only 0-6 whole genome multilocus sequence typing allele differences.
SPM-1-producing Pseudomonas aeruginosa: analysis of the ancestor relationship using multilocus sequence typing, pulsed-field gel electrophoresis, and automated ribotyping.
The first aim of the present study was to use spa typing and Multilocus sequence typing (MLST) typing to investigated the phylogenetic profile of Staphylococcus aureus strains from mastitis in dairy cows, the objective was also to better determinate the clonal complex of strains.
The multilocus sequence typing pattern of one highly positive sample showed a new allelic combination.
We attempted to develop an HRMA-based procedure to discriminate multilocus sequence typing (MLST) (2) alleles from a divergent lineage of Staphylococcus aureus (3) using a Corbett Rotorgene 6000.
Different molecular techniques such as pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify clonal groups of S.
alujai for Wolbachia infection using multilocus sequence typing and through assessment of the DNA sequence alignments, then determined the phylogenetic relationships amongst the bacteria identified.
The strains were divided into weak or strong adherent phenotypes and grouped using three molecular subtyping methods: multilocus sequence typing, pulsed-field gel electrophoresis and ribotyping.
Multilocus sequence typing of B burgdorferi in ticks suggested that both the ticks and the bacterium are most likely being introduced from the northeastern United States.
Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species.