Currently, the World Health Organisation (WHO) has endorsed molecular beacon
technology for the direct detection of MTB by using ultra-sensitive polymerase chain reaction (PCR) technology.
This decision is a major independent validation of Miacom's proprietary molecular beacon
technology being successfully applied in the hemoFISH assays.
Detection is by a fluorescently-labeled molecular beacon
The ANSR system uses an innovative isothermal DNA amplification process and fluorescent molecular beacon
technology for detection of the pathogen target.
A real-time PCR assay with 6 molecular beacon
probes was performed by using an iQ5 iCycler instrument (Bio-Rad, Hercules, CA, USA) to screen for mutations associated with isoniazid and rifampin resistance (19).
Next, 15 nanograms of the DNA was amplified using Brilliant QPCR Master Mix (Stratagene, La Jolla, CA) with gene-specific primers and each molecular beacon
on a Mx3000P Real-time PCR System (Stratagene) The oligonucleotides were as follows (shown in 5'-3' orientation): forward primer AAGAGTGGTTCTCGTTCTTACG, reverse primer GTGAGGGTGGGCGCAGAG, Allele C Beacon FAM-CCGGATCAGTTGTGTCTTCG CATCGTAAAGGACGATCCGG-BHQ1 and Allele G Beacon HEX-CCGGATCAGTTGTGTCTTC GGATCGTAAAGGACGATCCGG-BHQ1.
The sensitivity and accuracy of molecular beacon
and TaqMan[R] probe PCR assays were compared with the conventional USDA microbiological procedure using artificially contaminated RTE meats.
hybridization probes specific to mRNA targets will be conjugated to SERS NP-labels to allow optical detection utilising Raman microscopy in human cancer tissue sections.
probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA.
Scorpion probes build further on this idea by, in effect, using a molecular beacon
which also has one of the target PCR primers added to one end of the beacon region.
A novel ultrasensitive, molecular beacon
(MB) based sensing system for the mercury ion ([Hg.
The molecular beacon
fluorescence in situ hybridization (FISH) assay performed during the fourth infection trial used modified reverse PCR primer sequences for genogroup I and II viruses (28).