minisatellite DNA

minisatellite DNA

a form of polymorphic DNA, comprising a variable number of tandem repeats, with repeat units of up to about 100 NUCLEOTIDES in length, but typically 15–20bp. In humans, minisatellites form clusters up to about 5kb in length and are highly polymorphic due to the variation in repeat number. Telomeric DNA (see TELOMERE is an example of minisatellite DNA.

Minisatellite DNA is also called a VARIABLE NUMBER TANDEM REPEAT (VNTR).

The variability in repeat number at a specific VNTR LOCUS, makes analysis of minisatellite DNA useful for DNA FINGERPRINTING. The repeat region is often flanked by specific RESTRICTION ENZYME sites, so that it can be cut out of the chromosome and the number of repeats determined by a Southern blot (see SOUTHERN TRANSFER) and HYBRIDIZATION with a PROBE containing the repeat sequence. The DNA profile (fingerprint) generated can be individual-specific. If a number of different VNTR loci are analysed in this way the fingerprints become more discriminatory, making them particularly useful in DNA FORENSICS.

Another type of tandemly repeated DNA is the MICROSATELLITE. Microsatellite clusters are smaller than minisatellite clusters.

References in periodicals archive ?
The use of minisatellite DNA variation for stock identification of chum salmon, Oncorhynchus keta.
Smaller regions (made up of repeat units [less than]65 bp [base pairs]) are known as minisatellite DNA and can occur throughout the genome (Jeffreys et al.
Genetic variation at minisatellite DNA loci among North Pacific populations of steelhead and rainbow trout (Oncorhynchus mykiss).
With the DNA testM using two types of minisatellite DNA, the chance of a mistaken identification is less than 5 X 10.
Population structure and identification of North Pacific Ocean chum salmon (Oncorhynchus keta) revealed by an analysis of minisatellite DNA variation.
Population and species variation of minisatellite DNA in Plantago.
Minisatellite DNA is a class of repetitive nuclear DNA consisting of variable numbers of tandemly repeated copies (or VNTRs) of sequence that occur between restriction endonuclease restriction sites.
Initially, both mtDNA and minisatellite DNA techniques were used to evaluate population structure in the remaining colony, in some tissue recovered from the extinct colonies, and in the closely related southern hairy-nosed wombat (L.
1987), it provides additional information that cannot be ascertained from analysis of minisatellite DNA.
To assess whether neighbors were related, we analyzed minisatellite DNA banding patterns in 54 cicada killers that were nesting at various distances apart.
Restriction fragment profiles of hypervariable minisatellite DNAs (Jeffreys et al.