miniprep


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miniprep

A rapid, abbreviated method for identifying cloned DNA in a culture of host bacteria.

Steps in minipreps include lysis of host cells, cutting the cloned DNA/vector with a restriction endonuclease, and running the digest on an electrophoretic gel to identify characteristic bands.
References in periodicals archive ?
40 h at 37[degrees]C with shaking, the high-molecular weight bacmid DNA containing the assembled SINV-3 genome was purified using the Purelink Hipure Plasmid DNA Miniprep kit according to the manufacturer's instructions (Invitrogen).
Tenders are invited for Supply of qiagen kits for qiacube system and invitrogen reagents: qiaperp spin miniprep kit ( 1 kit: 50 rxn) (cat no.
Nucleic acid extraction was performed from 400 [micro]L of TRIzol-inactivated donor serum by using the Direct-zol RNA MiniPrep Kit (Zymo Research, Irvine, CA, USA), and on-column treatment was performed with Turbo DNase (Life Technologies, Carlsbad, CA, USA).
Recombinant colonies were subjected to plasmid isolation using the GeneJET Plasmid Miniprep Kit and confirmed by restriction endonuclease digestion, using EcoRI for the release of insert.
coli cells (XL 10-Gold, Stratagene) and purified using Quiagen's Miniprep kit according to the manufacturer's instructions.
Plasmids DNA of recombinant clones were isolated using QIAprep Spin miniprep kit (QIAGEN, Germany).
1 vector (Kpnl and Xbal sites) was cloned into a pAd5E3-CMV shuttle plasmid using ClaI and SpeI sites; the plasmids were then amplified by transfection into DH5[alpha] cells, and positive clones (pAd5E3-CMV-mBMP-2-pA) were selected and confirmed by DNA miniprep and PacI digestion.
The resultant PCR products were cloned using InsT/Aclone PCR cloning kit (Fermentas); plasmid-DNA was extracted using QIAprep Spin Miniprep Kit (Qiagen, CA, USA); and nucleotide sequenced using BigDye[TM] chemistry with M13-primers (Fermentas).
Genomic DNA was extracted from BM samples with the AxyPrep Blood Genomic DNA Miniprep Kit (Axygen Bioscience, USA) according to the manufacturer's protocol.
The Elute Mammalian Genomic DNA Purification Miniprep Kit (Sigma-Aldrichgen, G1N10) was used to isolate DNA from the tissue, The size of DNA was cut by the restriction enzymes Hpall and Mspl.
DNA was extracted from less than 30 mg of beetle body tissue following the Insect gDNA Miniprep Kit (BIOMIGA, Beijing, China) protocol.
Samples were homogenized using a Cuisinart Miniprep model DLC-1SS (Cuisinart Corp.