minigene

minigene

A popular term for a gene segment which encodes a variable region of either a heavy or light chain of an immunoglobulin.

minigene

a GENE that has been cloned and subjected to DELETION, by in vitro MUTAGENESIS, to reduce its size.
References in periodicals archive ?
Three four-day-old DGR dogs submitted to intravenous injection with an adenovirus-associated vector (AAV9) carrying a human codon with dystrophin minigene under the control of the cytomegalovirus (CMV), presented generalized muscle transgene expression after 16 weeks of treatment (KORNEGAY et al.
48) For in vitro functional evaluation of the exon skipping caused by the lower splicing efficiency of these variants, a minigene assay can be used.
The SOD2-knockout allele was screened by targeting amplification of the hprt minigene used to generate the knockout by using HPRTxl-2F (5'-CAGCCCTGGCGTCGTGAT TAGT-3') and HPRTx7R (5'-CGTGGGGTCCTTTTCACCAGCAA-3'), yielding a 2.
The use of gene therapy is to inject a smaller yet functional version of the gene - called a minigene - within the viral carrier.
A more practical minigene version would need to be only twenty nucleotides in length, but it would carry bits of code sufficient for the basic functions of initiating and stopping transcription.
A Multivalent minigene vaccine, containing B-cell, cytotoxic T-lymphocyte, and Th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen.
The majority of these used reverse transcriptase PCR (RT-PCR) [27] analysis of RNA extracted from blood of variant carriers or alternatively, minigene constructs containing the variant and assayed in non-patient-derived cell lines.
Knockout mice had been generated by removing portions of exons 2 and all of exons 3-5 of the Cyp1a2 gene; insertion of the Hprt minigene cassette was used for selection in embryonic stem (ES) cell cultures (Liang et al.
To confirm these in silico predictions we performed minigene expression studies.
A 'string-of-beads' vaccine, comprising linked minigenes, confers protection from lethal-dose virus challenge.
Relative contributions of VH and VL genes, minigenes, and complementarity-determining regions to binding of antibody-combining sites.