To determine if the RPL554 stimulation was mediated by inhibition of PDE3 or PDE4 in primary human bronchial epithelial cells (HBEs), and whether PDE inhibition was able to activate CFTR in CF airway epithelia, the specific PDE inhibitors Milrinone
(PDE3) and Rolipram (PDE4) were tested on HBEs from three R117H/F508del CF patients.
HNO also compared favorably in key hemodynamic parameters against the cAMP dependent therapy Milrinone
For the in vitro experiments, [+ or -]noradrenaline (NA) and milrinone
were obtained from Sigma, Germany.
Grand Island, NY) with 10 ng/mL epidermal growth factor (EGF), 10 milrinone
, 75 mg/L penicillin G, 50 mg/L streptomycin sulfate, 0.
He had a past medical history of hypertension, type 2 diabetes mellitus, severe ischemic cardiomyopathy with an ejection fraction of 15%, had been on home infusion therapy with milrinone
, and had been evaluated for orthotopic heart transplant.
The 33-year-old's debilitating dilated cardiomyopathy means he has to take 35 tablets a day, while an IV Milrinone
infusion continuously pumps drugs though his veins to keep him alive.
Papaverine, calcium antagonists (nimodipine, nicardipine or verapamil) and milrinone
(46,47) are effective in reversing cerebral vasospasm to varying extents (48) but repeat treatments are frequently required.
Hypertension is treated with milrinone
, a phosphodiesterase inhibitor.
(not freely available in South Africa) or dobutamine.
Many vasodilator agents such as papaverine, Ca antagonists, phosphodiesterase inhibitor- milrinone
, phenoxybenzamine and verapamil-nitro-glycerine, verapamil-papaverine solutions are used overcome the spasm.
Of these agents, the commonly used in clinical practice are dopamine, dobutamine, ephinephrine, norephinephrine, milrinone
CT angiography revealed vasospasm, and she went to IR for intra-arterial milrinone
(Primacor) and/or nicardipine (Cardene) treatment.