migration inhibition assay

migration inhibition assay

An immune function test that measures MIF (monocyte/macrophage inhibitory factor) and LIF (leukocyte inhibitory factor) production after lymphocyte stimulation with common antigens—e.g., streptokinase-streptodornase, Candida antigen, PPD, concanavalin A, phytohemagglutinin, pokeweed mitogen, etc.
 
In the MIA, peripheral lymphocytes are harvested from peripheral blood, spleen or lymph nodes, washed, and then separated from the plasma by centifugation; the cells are then exposed to the mitogen, washed again, and then incubated. The supernatent is then used to determine the inhibition of migration on a sample of naïve macrophages (e.g., from guinea pigs). The MIA correlates well with immune competence, and is one of the best tests for delayed-type hypersensitivity. MIF production is lost or markedly decreased in immunodeficiency states such as AIDS, DiGeorge syndrome and Wiskott-Aldrich syndrome.
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The candidates, later, were tested for functional activity using an in vitro cell migration inhibition assay and an in vivo tumor xenograft model in combination with carboplatin.

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