Concentrations of 5% peracetic acid also reduced viral titers to the LLOQ of the microtitration
assay when virus was in either dried cell culture medium (Figure 2, panel A) or wet blood (Figure 2, panel B).
Test Procedure: A 50 u l phosphate buffer saline was added in each well of the microtitration
plate was used in order to determine the biofilm formation under stressed conditions.
Yellow streptavidin-coated low-fluorescence microtitration
plates, wash buffer, and assay buffer were from Kaivogen Oy.
Two-fold serial dilutions of separated sera were prepared using normal saline in 96 wells, round bottom microtitration
plate (Titertek(tm) UK).
Pedicel was removed using fine-tipped scissors and placed in microtitration
plates sealed with laminated PVC film.
Direct enzyme immunoassay in microtitration
plate and test strip format for the detection of saxitoxin in shellfish.
In brief, the sample and horseradish peroxidase labeled monoclonal antibodies were added in one step to the monoclonal antibody-coated microwells of the microtitration
plate, using a sandwich technique.
The in vitro interactions between the antibiotics and the compounds from lichens were investigated by a two-dimensional checkerboard microdilution assay, using a 96-well microtitration
plates as previously described (Segatore et al.
Cytotoxicity was determined using the microtitration
of 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyl tetrazolium bromide (MTT) assay (Sigma, USA) as reported by Mosmann .
Anti-mycobacterial antibodies were detected by enzyme immunoassay utilizing microtitration
plates based on the A60 antigen extracted and purified from Mycobacterium bovis BCG13.
One type of antibodies was immobilized on the internal surfaces of the cells of the plates for microtitration