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microtitration

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microtitration
procedure for titrating substances such as antibiotics, antibodies or viruses in which small volumes are used, often 0.025 or 0.05 ml, using special delivery and transfer equipment referred to as diluters, droppers or micropipettes. Commonly performed in 96 well plastic plates.


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In this assay, 10 [micro]L of each RT-PCR product was mixed with 50 [micro]L of DELFIA assay buffer (PerkinElmer Finland Oy, Turku, Finland), added to 3 parallel microtitration wells coated with streptavidin (PerkinElmer Finland Oy), and incubated at room temperature for 30 min.
Direct enzyme immunoassay in microtitration plate and test strip format for the detection of saxitoxin in shellfish.
For the microtitration assay, cells in a log-growth phase were incubated with different dilutions of the sample for 48 h; at that time the samples were replaced by fresh growth medium for further 24 h (Ruffa et al.
 
 
 
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