Supplies diagnostic kits must include all related consumables (calibration kits, sheat fluid, microtiter
plates, foils on microtiter
plates, reagents, control materials and any other supplies needed for operation) to carry out the required tests according to the expected number of tests.
Protein microarrays are arrays of proteins or peptides that are immobilized onto a solid surface such as a glass slide, membrane, microtiter
plate, or other solid surface.
Method: Wells of microtiter
plates were coated with 100 (xL of asialofetuin II (100[micro]g/mL) over night at room temperature (RT).
Their topics include the quantitation of vitamins using microbiological assays in microtiter
formats, determining vitamin C in fortified food products using high-performance liquid chromatography and mass spectroscopy, and analyzing carotenoids.
20[degrees] C, cultivation of cells in 96 well microtiter
plates, and 50 ml spin tubes or shake flasks up to 6 liters.
The chips sit in small wells, known as microtiter
plates, and are covered with a patient's tumor cells.
6 mg per ml to 5 mg per ml--using a 96-well microtiter
Sera from immunized mice were also screened for formation of an immunometric assay with Eu-labeled mAb E146 using sheep antimouse- coated Maxisorp microtiter
plates as described elsewhere (9).
Assays were performed according to the method of Clarke and Casals, modified for the microtiter
system for each virus as previously described (6,7).
The Virtual Cytometer scanning system transfers the beads from microtiter
plates to a cell where they self align into randomly ordered arrays for readout.
The GeneChip HTA system adapts the same industry-standard GeneChip technology and content that Affymetrix uses in its cartridges to a standard 96-well microtiter
plate, which runs on an automated system built with off-the-shelf robotic components.
In summary, for the detection of antigen-specific IgG, 96-well microtiter
plates (flat-bottomed; Maxisorp, NUNC, Roskilde, Denmark) were coated overnight at 4[degrees]C with 100 [micro]L/well of the antigen in carbonate buffer, pH 9.