The experimental system included two microsyringe
pumps in which 10g of air-dried feed and solvent are pushed out with a constant flow rate.
sup] A microsyringe
was placed in the hole toward the right ventricle until the needle had penetrated and was withdrawn with cerebrospinal fluid.
Drugs were injected slowly with a Hamilton microsyringe
into one of the LV under stereotactic guidance (Brega: 1.
The following major instruments were used: stereotactic frame (RWD68002, Shenzhen RWD Life Science, Shenzhen, China), Morris water maze (built in-house by Guangxi Medical University), microsyringe
(Shanghai Anting Microsyringe
Factory, Shanghai, China); and electronic balance (ML204, Mettler-Toledo, Shanghai, China).
Added 1 [micro]L of lymphocyte cell suspension of each well of the test tray using a Hamilton 50 [micro]L repeating microsyringe
Five uL of aliquot with seven-day enriched cultures were transferred to the prepared oil-well coated regions using a microsyringe
The animals were restrained manually and a 30-gauge needle connected by polyethylene tubing to a 25 [micro]1 microsyringe
(Hamilton, Birmingham, UK) was inserted through the skin and between the vertebrae into the subarachnoid space of the L5-L6 spinal segments.
5[micro]L were gently placed on the substrate using a microsyringe
to measure the static contact angle.
Solutions were freshly prepared just before use and were injected by a Hamilton microsyringe
frugiperda using a graduated microsyringe
(ALSARAR et al.
4] M) and dried in vacuum was placed in a rectangular glass chamber, and a liquid drop was introduced onto the substrate through a microsyringe
16] STZ dissolved in normal saline was infused bilaterally into the cerebroventricles (5 [micro]L on each site and the final concentration of 3 mg/kg body weight) through microinjection pump, which was connected to a Hamilton microsyringe