microspectrophotometry


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mi·cro·spec·tro·pho·tom·e·try

(mī'krō-spek'trō-fō-tom' ĕ-trē),
A technique for characterizing and quantitating nucleoproteins in single cells or cell organelles by their natural absorption spectra (ultraviolet) or after binding stoichiometrically in selective cytochemical staining reactions, as in the Feulgen stain for DNA.
See also: cytophotometry.

mi·cro·spec·tro·pho·tom·e·try

(mī'krō-spek'trŏ-fō-tom'ĕ-trē)
A technique for characterizing and quantitating nucleoproteins in single cells or cell organelles by their natural absorption spectra (ultraviolet) or after binding stoichiometrically in selective cytochemical staining reactions, as in the Feulgen stain for DNA.

microspectrophotometry

(mī″krō-spĕk″trō-fō-tŏm′ĕ-trē)
Method for the histochemical study of substances present in cells, such as nucleic acid, based on absorption in the ultraviolet spectrum. This method permits quantitative and qualitative studies of certain cellular components.
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References in periodicals archive ?
Make and model determination of hit-and-run vehicles by reflectance microspectrophotometry, Journal of Forensic Sciences (1986) 31:464-470.
Comparison of minute smears of lipstick by microspectrophotometry and scanning electron microscopy/energy-dispersive spectroscopy, Journal of Forensic Sciences (1991) 36:366-375.
DNA microspectrophotometry of shoot apical meristem cell populations in Ceratopteris thalictroides (Filicales).
Spectral sensitivity of four species of fiddler crabs (Uca pugnax, Uca pugilator, Uca vomeris and Uca tangeri) measured by in situ microspectrophotometry.
Transmission and reflectance microspectrophotometry of inks, Journal of Forensic Sciences (1988) 33:1171-1184.
This module is intended to familiarize the trainee with the use of ultraviolet/visible light microspectrophotometry as a qualitative, quantitative and objective method of color analysis.
Nuclear DNA content in plants was previously determined by feulgen microspectrophotometry of root tip or shoot tip mitotic cells (Bennett and Smith, 1976).
Short wavelength pigments are sometimes not seen in microspectrophotometry because they only occur in the eighth retinular cell.
Chapter 18 Fiber Examination - UV/VIS Microspectrophotometry 18
Sodium and potassium in vertebrate cochlear endolymph as determined by flame microspectrophotometry.
In addition to producing a flat sample block for subsequent scanning electron microscopy analysis, sections may be cut for light microscopy, ultraviolet-visible microspectrophotometry, and infrared microspectroscopy.
These techniques include nuclear magnetic resonance (NMR) scanning and microspectrophotometry for cell wall analysis (Barton, 1985, 1991; Hartley et al.