D) Grocott's methenamine-silver
stain showing broad-based budding yeast.
The Grocott-Gomori methenamine-silver
nitrate stain allowed the better visualisation of chains of darkly pigmented, spheroidal, yeastlike organisms (Figure 3).
6) A variety of stains, including H & E, Grocott-Gomori methenamine-silver
nitrate, and PAS frequently reveal characteristic hyphal elements in tissue.
The histochemical stain results for acid-fast bacilli (Ziehl-Neelsen) and fungi (Gomori methenamine-silver
stain) were likewise negative.
Tissues were sectioned in 5-[micro]n slices and were subsequently stained with hematoxylin and eosin, Gram's, Ziehl-Neelsen, periodic acid--Schiff, and Grocott methenamine-silver
Ziehl-Neelsen and Brown and Brenn stains were again non-contributory, but a Grocott-Gomori methenamine-silver
2001); using special stains H & E (hematoxylineosin) or methenamine-silver
stain (Pirani, 1996).
Stains for acid-fast bacilli with Grocott-Gomori methenamine-silver
nitrate were negative.
stain of transbronchial and thoracic lymph node biopsy specimens from a hospitalized student revealed the presence of yeasts consistent with H.
Special staining by Grocott-Gomori methenamine-silver
4) The organisms are readily apparent with the use of special histochemical stains, such as Gridley fungus stain, periodic acid-Schiff (with or without diastase), and Gomori methenamine-silver
stain (Figure 3).
and acid-fast stains were negative for fungal and mycobacterial organisms, respectively.