The Carbapenemases Producing Enterobacteriaceae strains were screened by the Modified Hodge Test, and Metallo- (Beta) - Lactamase production was confirmed by the EDTA combined disc test.
Only 6% were identified as Carbapenemases Producing Enterobacteriaceae and 9 (75%) of them were Metallo- (Beta) -Lactamase producing strains, while 3 (25%) contained other enzymes.
Conclusion: Metallo- (Beta) -Lactamase producing Enterobacteriaceae species are causing problems in tertiary care hospitals.
Metallo- (Beta) -Lactamases (M L) constitute the molecular class B (sub-group B1 and B2) of Ambler classification and Group 3 according to the Bush-Jacoby-Medeiros functional classification.
Most common M (Beta) L families are the Verona integron- encoded metallo- (Beta) -lactamases ( VIM), Active on Imipenem (IMP), German Imipenemase (GIM), and Sao Paulo metallo- (Beta) -lactamases (SPM), which are located within a variety of integrons, where they are incorporated as gene cassettes.
Structure of apo- and monometalated forms of NDM-1 - A highly potent carbapenem hydrolyzing metallo- (Beta) -lactamase.
Metallo- (Beta) -lactamase- producing gram-negative bacilli laboratory based surveillance in cooperation with 13 clinical laboratories in the Kinki region of Japan.
Infections with VIM-1 metallo- (Beta) -lactamase-producing Enterobacter cloacae and their correlation with clinical outcome.