mercaptoethanol


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mer·cap·to·eth·a·nol

(mer-kap'tō-eth'ă-nol),
A commonly used reducing agent.

mercaptoethanol

A reducing reagent which contains a thiol group that breaks disulfide bonds in proteins, rendering them functionally effete.
References in periodicals archive ?
A subsequent releach with urea plus mercaptoethanol produced no activity to anti-sera.
Addition of mercaptoethanol to the urea leach produced, after two weeks, moderate reactions with anti-sera to sheep, human, guinea pig, and weak reactions with anti-sera to rabbit, dog, deer, turkey, all of which may be a generalized reaction with Protein G.
Adding mercaptoethanol to the urea leach produced moderate reactions to all anti-sera tested, probably a generalized reaction with Protein G.
After one day the 6M urea leach gave no reactions; the addition of mercaptoethanol for two days produced faint reaction to anti-sera to guinea pig, turkey, rabbit, and after 16 days the reactions increased slightly to weak.
25ng sodium selenite), Mercaptoethanol (10 [micro]M/ml), Gentamicin (50 [micro]g/ml).
Samples were assayed by measuring the inhibition of NADH oxidation by mercaptoethanol in the presence of EDTA and Mn, following the methods of Paoletti and Mocali (1990).
To further confirm the production of MBL by these organisms, DDST was performed using imipenem, ceftazidime along with 2- mercaptoethanol and EDTA as inhibitors for MBL.
5 mmol/L glucose, 50 [micro]mol/L mercaptoethanol, 100 mL/L fetal bovine serum, 2 mmol/L glutamine, 1 mmol/L sodium pyruvate, and 10 mmol/L HEPES and were used for experiments between the third and fifth passages.
Therefore, a sample buffer without mercaptoethanol must be used to detect the 2 nondissociated transferrin isoforms in CSF.
Mercaptoethanol treatment [10 [micro]L of a 1:10 dilution of mercaptoethanol (Merck cat.
Each sample (maximum of 40 mg) was cut into smaller pieces and homogenized for 30-40 s in 400 [micro]L of RLT lysis buffer containing mercaptoethanol with use of a polytron PT1200 homogenizer (Kinematica AG).