A disaccharide formed by the hydrolysis of raffinose by β-fructofuranosidase; also present in plant juices; consists of a galactosyl residue linked α 1,6 to a glucopyranose.
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Tenders are invited for Supply of Item Potassium phosphate dibasic 500G, Dried yeast extract 500G, Tween 80 500ml, Peptone powder 500G, Trichloro acetic acid 100G, midoblack 25G, Acetic acid 500M,L Mueller hinton agar 500G, Dextrose 50 discs per vial Vial, Maltose 50 discs per vial Vial, Sucrose 50 discs per vial Vial, Lactose 50 discs per vial Vial, Galactose 50 discs per vial Vial, Melibiose ,50 discs per vial Vial, Cellobiose 50 discs per vial Vial, Inositol 50 discs per vial Vial, Xylose50 discs per vial Vial, Raffinose 50 discs per vial Vial etc.
The strains in group A (ZZU 3 and 21) produced acid from galactose, melibiose, trehalose, D-raffinose, and D-turanose and could not grow in 6.
Carbohydrates for hemagglutination inhibition studies (D-glucose, D-fructose, D-galactose, lactose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, xylose, D-raffinose, melibiose, L-arabinose, D-mannose and D-fucose) and size-exclusion standards were obtained from Sigma-Aldrich.
The isolates were phenotypically characterized by means of macro/micro-morphological and physiological features, as fermentation of glucose, maltose and sucrose; assimilation of the following carbon sources: D-Glucose, D-Galactose, D-Ribose, D-Xylose, L-Arabinose, D-Arabinose, L-Rhamnose, Sucrose, Maltose, Trealose, Cellobiose, Salicin, Melibiose, Lactose,Raffinose, Inulin, Starch, Glycerol, Erythritol, Ribitol, D-Glucitol, D-Mannitol, myo-Inositol, Lactate, Citrate, Tween 20, N-acetylglucosamine; assimilation of the following nitrogen sources: Nitrate, Nitrite, Ethylamine, Lysine, Creatine and Creatinine; starch formation; urea hydrolysis; Diazonium Blue B reaction; growth at 40 and 50[degrees]C and 50% D-Glucose, 10% NaCl/16% NaCl and in different temperatures.
Acid was produced from glucose, mannitol, inositol, sorbitol, sucrose, melibiose, esculin, lactose, salicin, xylose, adonitol, saccharose, trehalose cellobiose, raffinose and arabinose.
Biochemical characteristics: P-galactosidase (ONPG), arginine dihydrolase (ADH), lysine decarboxylase (LDC), ornithine decarboxylase (ODC), citrate utilization (CIT), H2S production (H2S), urease (URE), tryptophane deaminase (TDA), Voges Proskauer (VP), sucrose (SAC), melibiose (MEL), indole production (IND), Rhammose (RHA), gelatinase (GEL), Glucose (GLU), mannitol (MAN), inositol (INO), sorbitol (SOR), amygdalin (AMY), arabinose (ARA), oxidase (OX).
The reactions test for fermentations (glucose, galactopyranoside, citrate, mannitol, inositol, sorbitol, rhamnose, melibiose, arabinose), decarboxylation (lysine, ornithine), reduction (hydrogen sulfide), hydrolysis (gelatin, urea) and indole/Vogues-Proskauer production.
API 20E[R] strips-API 20E[R] strips include enzymatic tests for fermentation or oxidation of glucose, mannitol, inositol, sorbitol, rhamnose, saccharose, melibiose, amygdalin, and arabinose, along with nitrate reduction to nitrite and nitrate reduction to nitrogen gas.
Melibiose, difructose anhydride III and difructose anhydride IV enhance net calcium absorption in rat small and large intestinal epithelium by increasing the passage of tight junctions in vitro.
In this case the marker (an enzyme) is the bacteria's new-found ability to digest the sugar melibiose.