5% agarose gels at 100 V for 40 min, and the presence or absence of amplified DNA of the expected product size (412 bp) was assessed by comparison with products obtained for positive controls (plasmid ligations of Marteilia type M DNA supplied by Isabelle Arzul, Laboratory for Mollusc Diseases and OIE Reference Laboratory for bonamiosis and marteiliosis
, IFREMER, France).
of oysters--an invasive disease of molluscs.
Nevertheless, the cultivation of mussels in deeper waters with the single longline floating method seems to have an advantage, in terms of marteiliosis
, over the hanging parks established in shallow waters (Karagiannis & Angelidis 2007).
occurs in numerous species of farmed bivalves around the world (Berthe et al.
ostreae), MSX disease (Haplosporidium nelsoni), marteiliosis
(Marteilia refringens), mikrocytosis (Mikrocytos mackini), and perkinsosis (Perkinsus marinus and P.