Predictive functional profiling of microbial communities using 16S rRNA marker gene
Some plant species, most notably grasses, are more amenable to transformation by particle bombardment, which generally involves cotransformation of two separate plasmids or DNA molecules, one for the gene of interest and one for the marker gene
We have used transgenic techniques to produce animals bearing a gene for the collagen [alpha]1(IV) chain containing a single point mutation at the proposed CBS in combination with marker genes
rol-6 (su1006) and Green fluorescent Protein (GFP).
Further, European Food Safety Authority has also reaffirmed that aadA gene encoding resistance against spectinomycin/streptomycin pose no threat to humans or to the environment, thus a potential selectable marker gene
for plant transformation (EFSA, 2009).
Yamanaka and his colleagues now describe online and in an upcoming Nature how they also inserted a novel marker gene
that singled out the completely reprogrammed stem cells.
For Agrobacterium tumefaciens-mediated transformation, strain LBA4404 harboring a T-DNA containing the translational fusion gene cry1Ab-1B driven by the maize ubiquitin constitutive promoter along with its first intron and untranslated exon (Christensen and Quail, 1996) and bar (coding for phosphinothricin acetyltransferase) as the selectable marker gene
, under the control of the 70S promoter (double enhanced constitutive 35S promoter from Cauliflower mosaic virus), (Fig.
The Commission says several member states then raised objections to the import of the maize "in terms of molecular characterisation, allergenicity, toxicity, inadequate monitoring plan, accidental spillage, presence of antibiotic resistance marker gene
and detection of the product".
The transfer of marker genes
to target cells depends on the function of the envelope protein; therefore, the titer of neutralizing antibodies against the envelope can be measured by a reduction in marker gene
The approval of this marker gene
was a regulatory blunder committed in the United States and elsewhere, as it is clear that kanamycin is still widely in clinical use, and the marker gene
confers resistance to new generation aminoglycosides as well (6).
PPL said each of the 5 piglets, born in the United States, has a foreign marker gene
in its DNA structure, a major step toward the production of genetically engineered pig organs for use in human transplantation.
The controversial technology requires the use of an antibiotic-resistant marker gene
, which confers resistance to certain antibiotics in the people who eat GE foods.