This induces an increased capacity for FAO that could be due to a decreased concentration of malonyl-CoA
, attenuating the inhibition of carnitine palmitoyltransferase I (CPT1) that catalyzes the entry of FAs in mitochondria, a rate-limiting enzyme of FAO (Assifi et al.
Furthermore, FASN encodes fatty acid synthase, which forms a complex homodimeric enzyme and plays an important role in biosynthesis of long chain fatty acids from acetyl coenzyme A (CoA) and malonyl-CoA
(Semenkovich et al.
CPT1 activity is tightly controlled by its inhibitor, malonyl-CoA
(step 4), which is regulated by AMP-activated kinase (AMPK).
Furthermore, CPP and 5-CQA decreased the nuclear active form of SREBP-1, acetyl-CoA carboxylase activity, and cellular malonyl-CoA
4] The first step of fatty acid synthesis is the formation of malonyl-CoA
by condensation of acetyl-CoA and CO[sub.
Liao and Dipple traced those effects to lower levels of a fat metabolite called malonyl-CoA
and an additional fat oxidation pathway.
3] to ADP, orthophosphate and malonyl-CoA
, catalysed by the enzyme acetyl-CoA carboxylase (E.
coli by increasing the intracellular pool of malonyl-CoA
through various metabolic and protein engineering approaches.
The ACC activity was expressed as nmol of malonyl-CoA
produced * [min.
The AMPK inhibits the activity of ACC, the rate-limiting enzyme involved in the production of malonyl-CoA
used for fatty acyl-CoA biosynthesis, and causes a reduction in this reaction, which stimulates CPT1 and reduces the flux of substrates in the fatty acid anabolic pathway (Carling et al.
Daniel Lane and colleagues have now focussed on the role of malonyl-CoA
in the signalling system in the brain (specifically the hypothalamus) that has inputs into the higher brain centres that determine feeding behaviour, most notably appetite.
24 mM NADPH, 30 [micro]M acetyl-CoA and 40-50 [micro]M malonyl-CoA
at 37[degrees]C, and then 10 [micro]l of homogenate added.