Direct observation of hemocytes revealed signs of swelling and loss of pseudopodia, whereas discrete lysosomes
containing neutral red were still apparent in the cytoplasm.
The activation thus occurs before transfer of the enzyme into lysosomes
, probably in the endoplasmic reticulum, as predicted experimentally (3).
We found that lysosomes
are actually highly dynamic and play a key role in the way inflammatory cells function.
are not merely trash containers," said Ana Maria Cuervo, M.
Instead of using mannose-6-phosphate (M6P) containing oligosaccharides to target the mannose-6-phosphate receptor and deliver proteins to lysosomes
, this proprietary technology uses a peptide tag to target the M6P receptor with high affinity.
They watched the receptor take copies of CXCL12 and dump them in the cells' lysosomes
, pockets for breakdown and recycling of molecules the cell no longer needs.
The second poster, entitled "Clustering of anthracycline-containing lysosomes
in drug-resistant MCF-7 cells in response to tesmilifene" is scheduled to be presented on Monday April 14, 2008 from 1:00 pm to 5:00 pm.
The other process is autophagy, in which membranes form around damaged mitochondria (the cell's power plants) and these membranes fuse with lysosomes
that destroys the contents.
Once trapped in a cancerous cell's membrane, Provecta causes the cell's lysosomes
(which contain digestive enzymes that cause cell destruction) to leak or rupture.
The probes specifically target lysosomes
, which act as cells' thermostats and waste processors and which have been linked to a variety of diseases.
The published data indicate that unlike other RNases, the ONCONASE endocytosis pathway is not targeted to lysosomes
These endosomes fuse with other naturally-occurring organelles termed lysosomes
, which contain enzymes that serve as the digestive system of the cell.