For example, 96 specimens and controls can be lysed
at one time.
After the incubation with asbestos fibers, cells were washed with PBS and lysed
in RIPA buffer (PBS, 1% Nonidet P-40, 0.
The stained cells were subsequently lysed
with 1X FACS[TM] Lysing Solution and the obtained leukocytes were resuspended in 1 mL of 1 % fixative (Formaldehyde, Electron Microscopy Sciences, Fort Washington, PA) after washing twice with 1X PBS/0.
The filtered samples were washed with 5 mL of water and then lysed
for ~20 s.
In a second study, Barry Komm and Mark Haussler, working at the University of Arizona College of Medicine in Tucson, inclubated lysed
nuclei of sarcoma cells from rat and human bone with highly radioactive estrogen.
This award was given in recognition for successful introduction of PathHunter(TM) cell based assay platform and company's overall work on intact and/or lysed
cell based assays.
All strains were untypeable, except for the O6 and O34 serotypes, which were lysed
by the phages specific for O6 (DR1) and O34 (DR2).
The stimulated cells were collected and lysed
at 3 hr of treatment.
Comparing a live probiotic and a lysed
one, in terms of immune system support, is like comparing a match to a blowtorch," said Dr.
The sample preparation technology covered by the patents provides several key advantages for extracting nucleic acid from a sample, including (1) concentration of the cells in the sample to be lysed
on a solid phase material, such as a filter; and (2) highly effective ultrasonic lysis of the concentrated cells.
Then cells were lysed
in 2 mL HBSS, the lysate was clarified, and a portion of it was used for the first passage.
Although these telomerase-specific CTLs effectively lysed
(destroyed) DCs that had been transfected with either TERT RNA or RNA from renal cancer cells, they did not lyse DCs transfected with RNA from several normal tissues that express telomerase transiently or at low levels, including bone marrow, normal renal epithelium, normal skin, or adrenal gland.