This assumption was tested using cell lysates
for apolipo-protein E (APOE) genotyping in a PCR protocol optimized for genomic DNA.
The eTag assays were used to detect VEGFR2 homodimers, VEGFR2 phosphorylation, and downstream pathway activation in whole cells lysates
6-Mercaptopurine (6-MP; 10 [micro]L; final concentration, 4 mmol/L) was added to 300 [micro]L of red cell lysates
containing 100 [micro]L of 0.
The 2470 can produce very high quality microarrays of a wide variety of biological materials, including those at the forefront of much current research - proteins, antibodies and cell lysates
were depleted of either intact antigen or the main heat shock proteins gp96, HSP90, HSP70 and calreticulin, which constitute more than 90 percent of the peptide-binding chaperones in cells.
The first assumption in erythrocyte folate analysis is that dilution of whole blood with hypotonic diluents produces complete hemolysis and, hence, the release of all folate; historically, 10-fold dilution of whole blood with ascorbic acid (AA) has been widely used to create lysates
Metabolon researchers analyze compounds or pellets and lysates
from cells already dosed with a compound.
The ACLARA eTag Assay System is an excellent solution, enabling the simultaneous profiling of 10s to 100s of toxicity genes, proteins and implicated signaling pathways, directly from cells or cell lysates
and across thousands of samples.
In addition, we determined the concentration range of total IL-8 measured in blood lysates
from healthy subjects.
Furthermore, to examine whether the specific 3'-terminal region is a determinant for the observed apoptosis, plasmids were transfected into RD cells, and cell lysates
were analyzed for caspase-3, -8, and -9 activities.
arrays are a type of reverse phase protein microarray that contains a collection of up to several hundred tumor samples on slide.
In addition to the initial 5,000 labeled human full-length protein standards, OriGene plans to add to its mass spectrometry offering of custom protein labeling, labeled transfected lysates
, and eventually labeled peptide standards for all human proteins.