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luciferin

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luciferin
(loosif´rin),
n a chemical substance present in certain luminous organisms that, when acted upon by the enzyme luciferase, produces a glow called
bioluminescence.

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A firefly's light is produced by a chemical reaction involving a special protein, a pigment called luciferin, and oxygen.
The plate was placed in a Lmax luminometer (Molecular Devices Corporation, Sunnyvale, CA, USA), and the following sequence of events was programmed for each well: a) injection of 100 [micro]L luciferin assay mix (Promega); b) 4-sec delay; c) light production measured over a 10-sec period.
The firefly genes enable cells to make an enzyme called luciferase, which emits a pale yellowish green light when combined with a similarly named substance, luciferin.
 
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