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luciferin |
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luciferin (loosif´ n a chemical substance present in certain luminous organisms that, when acted upon by the enzyme luciferase, produces a glow called bioluminescence. |
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| A firefly's light is produced by a chemical reaction involving a special protein, a pigment called luciferin, and oxygen. The plate was placed in a Lmax luminometer (Molecular Devices Corporation, Sunnyvale, CA, USA), and the following sequence of events was programmed for each well: a) injection of 100 [micro]L luciferin assay mix (Promega); b) 4-sec delay; c) light production measured over a 10-sec period. The firefly genes enable cells to make an enzyme called luciferase, which emits a pale yellowish green light when combined with a similarly named substance, luciferin. |
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