The comparison of luciferase
activities according to PTGS2 haplotypes was performed with the GraphPad Prism 5.
Particles releasing luciferin and coenzyme A were designed to accumulate in the extracellular space of the mesophyll, an inner layer of the leaf, while the smaller particles carrying luciferase
enter the cells that make up the mesophyll.
The glow could also be turned off by training the plants to stop the reaction between the particles using a luciferase
Cells were seeded in 3 parallel replicate 96-well plates for luciferase
activity testing and in one 96-well plate for MTT viability testing in medium containing 10% human serum.
reporter plasmid containing PTEN 3'-UTR (PTEN-luci-WT) or mutated PTEN 3'-UTR(PTEN-luci-MUT) was constructed.
and fluorescent protein technologies have been combined in ATP sensors that exploit bioluminescence resonance energy transfer (BRET).
Forty-eight hours after transfection, the cells were treated with MGDGs and an LXR or RXR agonist for 24 h, and then luciferase
activities were determined using a DualGlo Luciferase
Assay System (Promega Corp.
Considering that COREXIT is a mixture of solvents and surfactants that might compromise membrane integrity and also that components of fetal bovine serum (FBS) may have PPAR[gamma] agonist activity that would obscure testing, we determined whether a PPAR[gamma] ligand-binding domain-GAL4-UAS luciferase
system (Forman et al.
In order to investigate the putative mechanism underlying the role of miR-1 in tumor angiogenesis, a dual luciferase
experiment was performed for identification of the target of this miRNA.
Mouse Sohoh1promoter luciferase
reporter constructs: We analysed the upstream region of Sohlh1(GenBank Gene ID: 227631) -2000 bp to +500 bp using Berkeley Drosophila Genome Project Neural Network Promoter Prediction software (http:// www.
activities of infected cells were examined 72 h postinfection.
activity was measured via the Dual- Luciferase
(R) Reporter Assay System (Cat# E1910; Promega USA).