luciferase


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Related to luciferase: luciferin

luciferase

(lo͞o-sĭf′ə-rās′, -rāz′)
n.
An enzyme present in the cells of bioluminescent organisms that catalyzes the oxidation of luciferin and ATP, producing light.

luciferase

A mono-oxygenase enzyme (EC 1.13.12.7) that catalyses bioluminescent reactions, using luciferin as a substrate.

luciferase

an oxidizing enzyme that, acting on the bioluminescent substance luciferin, causes luminosity in glow-worms and other insects. see LUMINESCENCE.

luciferase

(loosif´ərās´),
n an enzyme present in certain luminous organisms that act to bring about the oxidation of luciferins; energy produced in the process is liberated as bioluminescence.
References in periodicals archive ?
Six HREs were synthesized, concatemerized and then cloned in tandem (head-to-tail orientation) into pBl-GL vectors (BD Biosciences Clontech, San Jose, CA) that has a downstream reporter of firefly luciferase gene: this vector was named as pHRE-Luc (Zheng et al.
We used the Student's t-test to determine the significance of difference in COX-2 induction and AP-1, NFAT, or NF[kappa]B activation in luciferase reporter assays among various groups.
The enzymatically released luciferin can be quantified by the luciferase reaction.
More important, says Frawley, his group's results establish that microinjection of luciferase plasmids can help investigators follow the activity of almost any gene in a single, normal cell for hours or even days.
Improved bioluminescent detection of acetate kinase using thermostable firefly luciferase.
Millar transferred the firefly's luciferase gene into the genome of the tiny weed Arabidopsis thaliana.
The new SwitchGear LightSwitch system includes a genome-wide collection of assay-ready GoClone[TM] promoter and 3'UTR luciferase reporters paired with optimized LightSwitch luciferase assay reagents.
Transfection of SH-SY5Y cells with APP promoter luciferase reporter vector (Reinhardt et al.
Luciferase activity in cells transfected with a luciferase reporter plasmid and treated with vehicle (-), 100 nmol/L E2, 1 [micro]mol/L atrazine (Atr), 10 [micro]mol/L ICI (ER antagonist), E2 + ICI, or Atr + ICI.
The cells were transfected with the luciferase reporter plasmids (800 ng/well) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Like other firefly luciferases Lampyris turkestanicus,the Iranian firefly luciferase, is a 62 kDa-monomer enzyme [2].