The sigmoid growth pattern in all cases showed that cells started their logarithmic phase of growth at 4th day of subculture and cells remained more active during that period.
The logarithmic phase started from 4th to 10th days and after that a straight line represents the stationary phase (Grosser and Gmitter Jr, 2011).
After 4 h, strain 2014103005 showed slow growth, accelerated upon approaching the logarithmic phase
donovani promastigotes obtained from logarithmic phase (191.
5 and 76[+ or -] 19 lig/ml, respectively) but also inhibited logarithmic phase L donovani promastigotes (127 [+ or -] 3.
Moreover, with alteration in culture condition such as carbon source and so on, production of secondary metabolites may be started at the logarithmic growth phase; that is to say, under this condition in logarithmic phase
, cell division will be stopped and subsequently antibiotic production will be occurred [35, 36].
It is generally held that during the logarithmic phase all the bacteria are alive and all are actively dividing.
However, ordinary broth culture has shown that the total number of organisms generally exceeds the number of viable organisms even during the logarithmic phase.
Under the laminar hood, the tested cells in the logarithmic phase (ISC55, ISC88, ISC90, ISC40 and ISC25) were sampled and each sample was poured in separated 1/5 ml micro-tubes and was centrifuged at 6000 g turn in 10 minutes and the scheme was discarded.
Under the laminar hood, the tested cells in the logarithmic phase (ISC55, ISC88, ISC90, ISC40 and ISC25) were sampled and were kept in Four at the temperature of 50 degrees Celsius for 24 hours.
The lipase activity shows maximum at its late logarithmic phase
or stationary phase.