To further compare levels of polymorphism across loci and species, the polymorphic information content (PIC; Botstein et al.
The Wilcoxon nonparametric signed-rank test was used to determine whether Gulf Menhaden had significantly higher PIC scores across all loci than the other Brevoortia species.
The relative power of assignment of Brevoortia gene loci was tested with the program Whichloci (Banks et al.
Amplification was performed by using BIOTAQ polymerase for 12 MIRU and 3 ETR loci and Diamond polymerase for 7 VNTR loci with the optimized parameters in method 1.
Population Genotyping in Metropolitan Setting with 2 Panels of VNTR Loci
The probes that linked the oligomer LOCI particles to the PCR-amplified DNA were specific for each of the two sensitizer particles and for the chemiluminescent particle (Fig.
After 5 min at 75 [degrees]C to complete DNA polymerization, the double-stranded amplicon was denatured at 95 [degrees]C for 2 min, cooled to 50 [degrees]C for 15 min to allow probe annealing to the amplified DNA, and finally cooled to 37 [degrees]C for 60 min to allow annealing of the probes to the LOCI oligomer particles.
has been licensed to some 120 out-of-state organizations.
For discrimination analysis, Hunter-Gaston diversity index (HGDI) was calculated as described (31) and used for comparison of the discriminatory power of VNTR typing for individual loci and for all loci taken together.
The codes for the 15 loci were expressed in the following order: MIRU 2, 4, 10, 16, 20, 23, 24, 26, 27, 31, 39, 40, ETR-A, ET-RB, ETR-C.
Boston University is pleased to continue as a partner in Loci, which is one of the most exciting and innovative cyberspace web sites aimed at the 18 to 24 year-old college students," said Brent Baker, dean of Boston University's College of Communication.
According to Collins, LOCI will now be structured as a "network" consisting of highly focused channels on the Web.